1: Eur J Pharm Sci. 2007 Apr;30(5):380-391. Epub 2007 Jan 13.

An improved in vitro model of human intestinal follicle-associated epithelium to
study nanoparticle transport by M cells.

des Rieux A, Fievez V, Theate I, Mast J, Preat V, Schneider YJ.

Universite catholique de Louvain, Unite de Pharmacie Galenique, Avenue E.
Mounier, 73-20, 1200 Brussels, Belgium; Laboratoire de Biochimie cellulaire,
Institut des Sciences de la Vie, Universite catholique de Louvain, Croix du Sud,
5, 1348 Louvain-La-Neuve, Belgium.

An alternative in vitro model of human follicle-associated epithelium (FAE) to
study nanoparticle transport mechanisms by M cells was developed and
characterized. The previous in vitro model of human FAE has been improved by
inverting inserts after Caco-2 cell seeding. Raji and M cells were identified
only in inverted co-culture cell monolayers by immunohistochemistry, confocal
microscopy, and electron microscopy. The M cell conversion rate evaluated by
scanning electron microscopy ranged between 15 and 30% of cells. Transport of
200nm carboxylated polystyrene nanoparticles was higher and more reproducible in
the inverted model. Nanoparticle transport was temperature-dependent, not
affected by the presence of EGTA or by potassium depletion, but inhibited by
EIPA or nystatin, suggesting that it occurs most likely by macropinocytosis. The
inverted model appears more physiologic, functional and reproducible than the
normally oriented model.

PMID: 17291730 [PubMed - as supplied by publisher]

Related Links

    Transport of nanoparticles across an in vitro model of the human intestinal
follicle associated epithelium. [Eur J Pharm Sci. 2005] PMID:15946828
    
    Identification of cell adhesion molecules in the human follicle-associated
epithelium that improve nanoparticle uptake into the Peyer's patches. [J
Pharmacol Exp Ther. 2006] PMID:16914557
    
    Helodermin-loaded nanoparticles: Characterization and transport across an in
vitro model of the follicle-associated epithelium. [J Control Release. 2007]
PMID:17292503
    
    Transport of chitosan microparticles for mucosal vaccine delivery in a human
intestinal M-cell model. [J Drug Target. 2002] PMID:12575734
    
    Cell culture modeling of specialized tissue: identification of genes
expressed specifically by follicle-associated epithelium of Peyer's patch by
expression profiling of Caco-2/Raji co-cultures. [Int Immunol. 2004]
PMID:14688064