1: Skin Pharmacol Appl Skin Physiol 1998 Jan-Feb;11(1):23-34
In vivo noninvasive evaluation of hairless rat skin after high-voltage
pulse exposure.
Vanbever R, Fouchard D, Jadoul A, De Morre N, Preat V, Marty JP
Unite de Pharmacie Galenique, Universite catholique de Louvain, Bruxelles,
Belgique.
Short high-voltage pulses have recently been shown to dramatically increase
and expedite transdermal drug transport via a mechanism hypothesized to
involve electroporation. This study addresses tolerance issues of the method
in vivo in hairless rat. Chromametry, transepidermal water loss (TEWL),
laser Doppler flowmetry (LDF) and corneometry were jointly used for noninvasive
sensing of skin biophysical parameters. Slight increases in skin redness,
TEWL and LDF values followed the application of electric pulses. The changes
in skin capacitance were nonsignificant. The magnitude of the alterations
depended on the electrical features of the pulses. When compared to iontophoresis,
high-voltage pulses did not induce stronger alterations of skin functions.
This report provides the first in vivo demonstration of the safety of the
high-voltage pulses proposed for transdermal delivery.
PMID: 9603666, UI: 98261789