1: Bioconjug Chem  2001 May-Jun;12(3):451-7

Incorporation of an aldehyde function in oligonucleotides.

Tilquin JM, Dechamps M, Sonveaux E.

Laboratoire de Chimie Therapeutique et de Radiopharmacie, Universite Catholique
de Louvain, 73, Avenue Emmanuel Mounier, p. b. CMFA 7340, B-1200 Bruxelles,
Belgium.

A nucleotide-like phosphoramidite building block that has the nucleic base
replaced by the tert-butyldimethylsilyl-protected styrene glycol was
synthesized. After the automatic synthesis of an oligonucleotide incorporating
this synthon, the benzaldehyde function was generated by fluoride deprotection
and oxidation by sodium periodate. In a similar manner, an oligonucleotide where
a nucleic base was replaced by the (CH2)8CH=O chain was synthesized and
conjugated with biotin derivatives.

PMID: 11353544 [PubMed - indexed for MEDLINE]