1: J Synchrotron Radiat. 2005 May;12(Pt 3):292-8. Epub 2005 Apr 14. 

Radiolysis of proteins in the solid state: an approach by EPR and product
analysis.

Terryn H, Deridder V, Sicard-Roselli C, Tilquin B, Houee-Levin C.

Universite Catholique de Louvain, Faculte de Medecine, Departement de Pharmacie,
Unite CHAM 72.30, Bruxelles, Belgium.

Radio-induced modifications in proteins have been studied using several
techniques. Electron paramagnetic resonance (EPR) was used to characterize free
radicals, and analysis methods (high-performance liquid chromatography,
capillary electrophoresis) were employed to visualize final degraded forms.
Whereas EPR indicates that perthiyl radicals are formed, analysis does not
detect any compound in which such bonds would be broken. Since EPR signals decay
with time, it is concluded that rearrangements occur at subsequent steps, in
which the solvent used during the analysis might play a role.

PMID: 15840913 [PubMed - indexed for MEDLINE]