1: Life Sci.  2004 Jul 9;75(8):955-67.  

Inhibition of the development of metastases by dietary vitamin C:K(3)
combination.

Taper HS, Jamison JM, Gilloteaux J, Summers JL, Calderon PB.

Unite de Pharmacocinetique, Metabolisme, Nutrition, et Toxicologie, Universite
Catholique de Louvain, Avenue E. Mounier, 73, B-1200 Bruxelles, Belgium.

The tumor growth-inhibiting and chemo-potentiating effects of vitamin C and
K(3)combinations have been demonstrated both in vitro and in vivo. The purpose
of this study was to investigate the influence of orally administered vitamin C
and K(3) on the metastasis of mouse liver tumor (T.L.T.) cells implanted in C3H
mice. Adult male C3H mice were given water containing vitamin C and K3 (15
g/0.15 g dissolved in 1000 ml) beginning 2 weeks before tumor transplantation
until the end of the experiment. T.L.T. cells (106) were implanted
intramuscularly in the right thigh of mice. All mice were sacrificed 42 days
after tumor transplantation. Primary tumor, lungs, lymph nodes and other organs
or tissues suspected of harboring metastases were macroscopically examined.
Samples of primary tumors, their local lymph nodes, lungs and main organs such
as liver, kidneys, spleen were taken for histological examination. Forty-two
percent of control mice exhibited lung metastases and 27% possessed metastases
in local lymph nodes whereas 24% of vitamin-treated mice exhibited lung
metastases and 10% possessed local lymph nodes metastases. The total number of
lung metastases was 19 in control group and 10 in vitamin C and K(3)-treated
mice. Histopathological examination of the metastatic tumors from the
vitamin-treated mice revealed the presence of many tumor cells undergoing
autoschizic cell death. These results demonstrate that oral vitamin C and K(3)
significantly inhibited the metastases of T.L.T. tumors in C3H mice. At least a
portion of this inhibition was due to tumor cell death by autoschizis.

PMID: 15193956 [PubMed - in process]