1: Planta Med. 2004 Aug;70(8):764-70. 

Development and validation of a high performance liquid chromatographic method
for quantitative determination of aporphine alkaloids from different samples of
Cassytha filiformis.

Stevigny C, Wautier MC, Habib Jiwan JL, Chiap P, Hubert P, Quetin-Leclercq J.

Laboratoire de Pharmacognosie, Unite d'Analyse Chimique et Physico-Chimique des
Medicaments, Universite Catholique de Louvain, UCL 72.30-CHAM, Bruxelles,
Belgium.

A sensitive and accurate procedure based on an alkaloid extraction coupled to an
HPLC-UV-MS determination has been developed for the separation and
quantification of the major aporphines in Cassytha filiformis. The extraction
step and the liquid chromatography conditions were optimized in order to improve
the selectivity of the method. The HPLC mobile phase consisted of a mixture of
water containing 10 mM ammonium acetate adjusted to pH 3 with acetic
acid-acetonitrile (90: 10, v/v) (A) and acetonitrile (B) used in a gradient mode
(0 to 40%). The stationary phase was an RP-select B (5 micro m) column. The
method was completely validated using cassythine, one of the major aporphines in
our samples, as reference standard and successfully applied to the determination
of these pharmacologically interesting aporphines in seven different batches of
C. filiformis. The detection and quantitation limits of cassythine were found to
be 13 and 20 micrograms/mL, respectively. The results showed variations in the
total alkaloid content in samples from 0.11 to 0.43%.

Publication Types:
    Validation Studies

PMID: 15368674 [PubMed - indexed for MEDLINE]