TI: Diagnostic specificities and sensitivities of
anti dsDNA, anti membrane DNA and anti nucleosomes autoantibodies.
AU: Servais,-G; Daens,-S; Guillaume,-M-P; Cumps,-J; Duchateau,-J;
Peretz,-A
AD: Immunology Department, CHU Brugmann-Free University of Brussels,
Belgium. genevieve.servais@chu-brugmann.be
SO: Scand-J-Clin-Lab-Invest-Suppl. 2001; (235): 61-7
JN: Scandinavian-journal-of-clinical-and-laboratory-investigation.-Supplementum
PY: 2001
AB: The aim of this study is to evaluate, from 369 routine sera
of SLE and control patients, the worth of anti double stranded nuclear
DNA, anti nucleosomes autoantibodies and anti membrane DNA for the diagnosis
of SLE. Cell membrane associated DNA (mDNA) has been described on B lymphocytes
and monocytes, but not on T cells. Antibodies to mDNA were identified by
an indirect immunofluorescence assay using a B cell line fixed but not
permeabilised. At a 1:40 serum dilution, anti mDNA is almost associated
with the diagnosis of systemic lupus erythematosus (SLE). Anti mDNA were
shown to be different in specificity as compared with anti double stranded
nuclear DNA. We compare its characteristics as diagnostic procedure to
the conventional anti dsDNA antibody detection and to the recently introduced
anti nucleosome antibody test documented as associated with SLE. It appears
that the best sensitivity (0.65) and specificity (0.98) is given by the
anti mDNA test.