1. J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 1;877(23):2396-402. Epub 
2009 Mar 19.

An improved HPLC-UV method for the simultaneous quantification of triterpenic
glycosides and aglycones in leaves of Centella asiatica (L.) Urb (APIACEAE).

Rafamantanana MH, Rozet E, Raoelison GE, Cheuk K, Ratsimamanga SU, Hubert P,
Quetin-Leclercq J.

Institut Malgache de Recherches Appliquées, BP 3833, 101 Antananarivo,
Madagascar. mamy.rafamantanana@uclouvain.be

The simultaneous quantification of madecassoside, asiaticoside, madecassic acid
and asiatic acid in Centella asiatica by HPLC-UV is proposed. Asiaticoside was
used as reference for the quantification of heterosides and asiatic acid for
aglycones. The evaluation of the extraction efficiency of the four molecules led 
to use Soxhlet extraction for 8 h. The method was validated and was found to be
accurate in the concentration range of 1.0-3.0 mg/ml for asiaticoside and 0.5-2.0
mg/ml for asiatic acid with CV <3% for all investigated compounds. LOD and LOQ
were, respectively, 0.0113 and 1.0 mg/ml for asiaticoside and 0.0023 and 0.5
mg/ml for asiatic acid. This method was shown to be convenient for routine
analysis of samples of C. asiatica.

PMID: 19349219 [PubMed - indexed for MEDLINE]