1. J Pharm Biomed Anal. 2009 Dec 5;50(5):753-63. Epub 2009 Jun 16.

Development of HPLC fingerprints for Mallotus species extracts and evaluation of 
the peaks responsible for their antioxidant activity.

Nguyen Hoai N, Dejaegher B, Tistaert C, Nguyen Thi Hong V, Rivière C, Chataigné
G, Phan Van K, Chau Van M, Quetin-Leclercq J, Vander Heyden Y.

Department of Analytical Chemistry and Pharmaceutical Technology, Vrije
Universiteit Brussel (VUB), Laarbeeklaan 103, B-1090 Brussels, Belgium.

Some Mallotus species are used in traditional medicine in Vietnam. To use certain
species in Western medicines or as food supplements, they should be identified
and quality control should be more strict, for instance, to avoid the erroneous
switching of species. In species with interesting activities, the compounds
responsible for them should be identified. For these identifications, HPLC
fingerprint methodology can be used. In this paper, HPLC fingerprints of
different lengths were developed for a number of Mallotus species. Secondly, a
multivariate regression model was constructed to model the antioxidant activity
of the Mallotus samples from the HPLC fingerprints with the aim to indicate peaks
possibly responsible for this activity. For this purpose, after data
pretreatment, the calibration technique partial least squares (PLS) was applied.

PMID: 19586736 [PubMed - indexed for MEDLINE]