1: C R Seances Soc Biol Fil 1995;189(4):643-56
[In vitro study of the metabolic conversion of aflatoxin B1 into its epoxide].
[Article in French]
Mukendi N, de Meester C, Rollman B
The conversion of aflatoxin B1 into its epoxide was evaluated in vitro
by two different approaches based on HPLC analysis: the quantitative estimation
of the tris(OH)AFB1 addition product resulting from the indirect reaction
of AFB1-epoxide with tris buffer (hydroxymethyl) aminomethane and on the
other hand by the quantification of the formation of the glutathione conjugate
(AFB1-SG). The tris(OH)AFB1 is more sensitive than AFB1-SG in fluorescence
detection. The AFB1-SG obtained (5.42 +/- 0.42 microgram) is weakly less
than the quantity of tris(OH)AFB1 (6.00 +/- 0.72) obtained in the same
experimental conditions.
PMID: 8564579, UI: 96149272