1: J Appl Toxicol 1998 Mar-Apr;18(2):103-9
Thermic transition and glycolytic capacity as critical events in the survival
of rat liver slices after overnight cold hypoxic preservation.
Morales H, Taper H, Buc Calderon P
Departement des Sciences Pharmaceutiques, Universite Catholique de Louvain,
Bruxelles, Belgium.
Cellular survival and hypoxia-reoxygenation injury in overnight cold-preserved
liver slices (+/-20 h at 4 degrees C) were investigated. Increased cell
death after overnight cold hypoxia depended more on temperature than on
the reoxygenation process itself. Fructose (at 50 mM) added before the
onset of hypoxia improved survival at the end of 20 h of cold hypoxia over
Krebs- or glucose-treated slices. Such a protective effect by fructose
was also seen during the normothermic (37 degrees C) reoxygenation of previously
cold hypoxic-preserved slices, but only in the absence and not in the presence
of tert-butyl hydroperoxide, a model compound widely used to induce an
oxidative stress. The protection by fructose was equivalent to that observed
when liver slices were incubated in the University of Wisconsin solution
(UW). Finally, the morphological study of haematoxylin and eosin (H &
E)-stained slices has shown cytoplasmic vacuoles during the reoxygenation
step, which were more pronounced in UW-treated than in fructose-treated
slices.
PMID: 9570692, UI: 98230619