1: Vaccine. 2008 May 2;26(19):2360-8. Epub 2008 Mar 18.

Safety evaluation of pulmonary influenza vaccination in healthy and "asthmatic"
mice.

Minne A, Huaux F, Jaworska J, Rha RD, Hamelmann E, Vanbever R.

Department of Pharmaceutical Technology, Université catholique de Louvain, Avenue
E. Mounier, 73 UCL 73.20, Brussels B-1200, Belgium.

The present study reports animal immuno-toxicological data of pulmonary
vaccination against inactivated seasonal influenza. Its aims were (i) to monitor 
the temporal kinetics of lung inflammation in normal mice over a period of 2
weeks following pulmonary vaccination in order to assess the risk of chronic lung
inflammation, (ii) to evaluate the impact of pulmonary vaccination on the
asthmatic phenotype in an established allergen-sensitized murine model of asthma.
Both sets of experiments were performed using high doses of split influenza virus
vaccine. In the first part of this study, we showed that pulmonary influenza
vaccination induced a slight local inflammatory response which was limited in
duration since it was no longer observed at 2 weeks post-vaccination. At this
time point, it has previously been shown that the immunogenic efficacy was
maintained. In the second part, we demonstrated that pulmonary influenza
vaccination did not significantly exacerbate the cardinal features of asthma,
i.e., allergen-specific IgE formation, the development of airway hyperreactivity 
(AHR) and eosinophilic airway inflammation. Our data therefore suggest that the
overall immuno-toxicological profile of pulmonary vaccination against seasonal
influenza was acceptable, even in an animal model of pulmonary hypersensitivity.


PMID: 18403071 [PubMed - in process]

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