1: FASEB J. 2008 Mar 7. [Epub ahead of print]

Gut microbiota modulation with norfloxacin and ampicillin enhances glucose
tolerance in mice.

Membrez M, Blancher F, Jaquet M, Bibiloni R, Cani PD, Burcelin RG, Corthesy I,
Macé K, Chou CJ.

*Nestlé Research Center, Lausanne, Switzerland;Université Catholique de Louvain, 
Unit of Pharmacokinetics, Metabolism, Nutrition and Toxicology, Brussels,
Belgium; andInstitute of Molecular Medicine Rangueil (12 MR), INSERM U858, IFR31,
Toulouse, France.

Recent data suggest that the gut microbiota plays a significant role in fat
accumulation. However, it is not clear whether gut microbiota is involved in the 
pathophysiology of type 2 diabetes. To assess this issue, we modulated gut
microbiota via antibiotics administration in two different mouse models with
insulin resistance. Results from dose-determination studies showed that a
combination of norfloxacin and ampicillin, at a dose of 1g/L, maximally
suppressed the numbers of cecal aerobic and anaerobic bacteria in ob/ob mice.
After a 2-wk intervention with the antibiotic combination, both ob/ob and
diet-induced obese and insulin-resistant mice showed a significant improvement in
fasting glycemia and oral glucose tolerance. The improved glycemic control was
independent of food intake or adiposity because pair-fed ob/ob mice were as
glucose intolerant as the control ob/ob mice. Reduced liver triglycerides and
increased liver glycogen correlated with improved glucose tolerance in the
treated mice. Concomitant reduction of plasma lipopolysaccharides and increase of
adiponectin further supported the antidiabetic effects of the antibiotic
treatment in ob/ob mice. In summary, modulation of gut microbiota ameliorated
glucose tolerance of mice by altering the expression of hepatic and intestinal
genes involved in inflammation and metabolism, and by changing the hormonal,
inflammatory, and metabolic status of the host.-Membrez, M., Blancher, F.,
Jaquet, M., Bibiloni, R., Cani, P. D., Burcelin, R. G., Corthesy, I., Macé, K.,
Chou, C. J. Gut microbiota modulation with norfloxacin and ampicillin enhances
glucose tolerance in mice.


PMID: 18326786 [PubMed - as supplied by publisher]

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