TI: Assessment of [18F]fluoroethylflumazenil metabolites
using high-performance liquid chromatography and tandem mass spectrometry.
AU: Leveque,-P; de-Hoffmann,-E; Labar,-D; Gallez,-B
AD: Unite de Chimie Pharmaceutique et de Radiopharmacie, Universite
Catholique de Louvain, Brussels, Belgium.
SO: J-Chromatogr-B-Biomed-Sci-Appl. 2001 Apr 15; 754(1): 35-44
PY: 2001
AB: A simple procedure using HPLC and tandem mass spectrometry
has been developed for the determination of fluoroethylflumazenil metabolites.
Samples were precipitated with acetonitrile, evaporated to dryness followed
by reconstitution with methanol. As mobile phase, 50 mM ammonium formate-methanol
(58:42, v/v) was used. The method is valid both for cold and radiolabelled
metabolites. Various cold metabolites (hydroxylated and/or dealkylated)
were identified in rat and human microsome preparations. Radiolabelled
metabolites arise from two or more transformations including hydroxylation.
The methodology developed can be applied for further characterisation of
metabolites, and for the determination of non metabolised [18F]fluoroethylflumazenil
in routine clinical analysis.