ARTICLE TITLE: A qualitative method for assessing the C-14 labelling position in [C-14]-dimethyl aminoantipyrine (aminopyrine)

ARTICLE AUTHOR: Leveque-P; Gallez-B REPRINT AUTHOR: Leveque, P; UNIV CATHOLIQUE LOUVAIN; DEPT PHARMACEUT SCI, LAB MED CHEM AND RADIOPHARM, UCL-CMFA 73-40; B-1200 BRUSSELS; BELGIUM

SOURCE: JOURNAL-OF-LABELLED-COMPOUNDS-AND-RADIOPHARMACEUTICALS. OCT 1998; 41 (10) : 959-963

ABSTRACT:

[C-14]-Aminopyrine is a well known compound which is widely used for the evaluation of the hepatic function (breath test). As this breath lest is based upon N-demethylation, it is essential to verify the labelling position in order to guarantee relevant results. We propose an enzymatic method based on collection of (CO2)-C-14 produced after specific in vitro N-demethylation in a medium containing horseradish peroxidase, formaldehyde dehydrogenase and formate dehydrogenase. This assay allows for a qualitative monitoring of the labelling position.