Intranasal immunization against influenza virus using polymeric particles.
Lemoine D, Deschuyteneer M, Hogge F, Preat V
Universite catholique de Louvain, Unite de Pharmacie Galenique, Brussels,
Belgium.
The aim of this study was to evaluate the potential of poly(D,L-lactide-co-glycolide)
nano-and microspheres, with a mean diameter of 220 nm and 8 microm, respectively,
to enhance the nasal and systemic immune responses against influenza virus
antigen. High encapsulation levels of antigen were achieved in all cases.
Neither the molecular weight nor the antigenicity of the entrapped antigen
were affected by the encapsulation procedure. Following nasal immunization,
the nasal washes IgA and the serum IgG responses were evaluated. With the
soluble antigen, relatively high immune responses were observed. With nanospheres,
nasal washes IgA levels were significantly lower (p<0.01) and serum
IgG levels were not significantly different (p>0.05) from those obtained
with the soluble antigen. With microspheres, both nasal washes IgA and
serum IgG levels were significantly lower (p<0.01 and <0.05, respectively)
as compared to the levels found for the soluble antigen. In addition, fluorescent
microspheres administered intranasally failed to reach the nasal-associated
lymphoid tissue (NALT). This lack of particle uptake by NALT and the high
immunogenicity of the antigen used in this study, could explain the absence
of enhancement of the immune responses by the polymeric particles.
PMID: 10487316, UI: 99415238