ARTICLE AUTHOR: Lemoine-D; Wauters-F; Bouchend'-homme-S; Preat-V

REPRINT AUTHOR: Preat, V; Univ Catholique Louvain; Unite Pharm Galen; Ave E Mounier 73-20; B-1200 Brussels; Belgium

SOURCE: INTERNATIONAL-JOURNAL-OF-PHARMACEUTICS. DEC 30 1998; 176 (1) : 9-19

ABSTRACT:

The goal of this study was to evaluate the technological feasibility of delivering antigen using alginate microspheres. The microspheres were prepared by an emulsification technique and fully characterized as antigen delivery system. Selection of appropriate parameters enabled the preparation of alginate microspheres with a mean diameter of 8 mu m. The encapsulation efficiency of bovine serum albumin (BSA), chosen as model antigen, as well as the BSA loading were very high (>90% and 10% w/w, respectively). The process of encapsulation did not affect the molecular weight or the antigenicity of the entrapped antigen. The in vitro release profile showed a fast release rate of encapsulated BSA, particularly in phosphate buffered saline solution. However, a decrease of the release rate was observed when alginate microspheres were coated with poly(L-lysine) or prepared with higher alginate molecular weight. Therefore, alginate microspheres appear, technologically, a promising antigen delivery system. (C) 1998 Elsevier Science B.V. All rights reserved.