Test for antioxidant ability by scavenging long-lived mutagenic radicals in mammalian cells and by blood test with intentional radicals: an application of gallic acid

Kumagai-J; Kawaura-T; Miyazaki-T; Prost-M; Prost-E; Watanabe-M; Quetin-Leclercq-J RP: Kumagai, J;
Nagoya Univ; Grad Sch Engn; Furo Cho; Nagoya; Aichi 4648603; Japan IN: Nagoya Univ, Grad Sch Engn, Dept Appl Chem, Chikusa Ku, Nagoya, Aichi 4648603, Japan; CEDRA, F-21560 Couternon, France; Nagasaki Univ, Sch Pharmaceut Sci, Nagasaki 8528521, Japan; Univ Catholique Louvain, Fac Med, Sch Pharm, Cham Unit, B-1200 Brussels, Belgium EM: Kumagai, J: kumagai@apchem.nagoya-u.ac.jp

RADIATION-PHYSICS-AND-CHEMISTRY. JAN 2003; 66 (1) : 17-25 DT:

Antioxidant ability of gallic acid (GA) are determined both by electron spin resonance measurement of long-lived radicals produced in gamma-ray irradiated Syrian golden hamster embryo cells with GA and by hemolysis measurement with GA when blood cells are submitted to radicals. Scavenging properties of GA are determined by the reaction rate constant with long-lived mutagenic radicals in the cells while the blood test allows to analyze the global effects of this compound: radical scavenger + metal ion chelator + regeneration of intra- and extra-cellular antioxidant.