1. J Pharm Biomed Anal. 2008 Dec 1;48(4):1127-35. Epub 2008 Sep 18.

Validation of a method for the determination of sterols and triterpenes in the
aerial part of Justicia anselliana (Nees) T. Anders by capillary gas
chromatography.

Kpoviéssi DS, Gbaguidi F, Gbénou J, Accrombessi G, Moudachirou M, Rozet E, Hubert
P, Quetin-Leclercq J.

Pharmacognosy and Essential Oils Laboratory, Faculty of Health Science (FSS),
Faculty of Sciences and Technics (FAST), University of d'Abomey-Calavi (UAC), 01 
BP 188 Cotonou, Benin. 30-CHAM, Av Mounier 72, B-1200 Bruxelles, Belgium.

An accurate and sensitive method, combining soxhlet extraction, solid
phase-extraction and capillary gas chromatography is described for the
quantitative determination of one triterpene (lupeol) and three sterols
(stigmasterol, campesterol and beta-sitosterol) and the detection of another
triterpene (alpha-amyrin) from the aerial part of Justicia anselliana. This is
the first method allowing the quantification of sterols and triterpenes in this
plant. It has been fully validated in order to be able to compare the sterol and 
triterpene composition of different samples of J. anselliana and therefore help
to explain the allelopathic activity due to these compounds. This method showed
that the aerial part of J. anselliana contained (292+/-2)mg/kg of lupeol,
(206+/-1)mg/kg of stigmasterol, (266+/-2)mg/kg of campesterol and (184+/-9)mg/kg 
of beta-sitosterol.

PMID: 18951746 [PubMed - indexed for MEDLINE]