1: Magn Reson Med. 2009 Mar;61(3):634-8.

Rapid monitoring of oxygenation by 19F magnetic resonance imaging: Simultaneous
comparison with fluorescence quenching.

Jordan BF, Cron GO, Gallez B.

Laboratory of Biomedical Magnetic Resonance, Université Catholique de Louvain,
Brussels, Belgium.

The aim of this study was to develop an MRI fluorocarbon oximetry technique using
snapshot inversion recovery and compare it with fluorescence quenching
fiber-optic probe oximetry (OxyLite) performed simultaneously in experimental
mouse tumors. The oxygen reporter probe hexafluorobenzene (HFB) was injected
directly into the tumors, along with the insertion of the OxyLite probe. Tumor
oxygenation (pO(2)) was modified using carbogen or lethal doses of the anesthetic
gas. MRI pO(2) maps were generated in 1.5 min with an in-plane spatial resolution
of 1.88 mm. MRI and OxyLite showed consistent baseline and postmortem pO(2)
values. Increases in tumor pO(2) during carbogen breathing showed similar
kinetics for the two methods. The pO(2) values observed using the OxyLite
corresponded with relatively hypoxic values observed by MRI. The apparent
discrepancy between mean values might be due to the difference in sampling
volumes of the techniques and the observation of multiple locations using (19)F
MRI versus a single location using the large optical fiber. Overall, the present 
method provides a rapid way to map the tumor oxygenation and is particularly
suitable to monitor acute changes of pO(2) in tumors.


PMID: 19097235 [PubMed - in process]

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