1: Pharm Res. 2008 Jan;25(1):87-103. Epub 2007 Nov 6. In vivo methods for the assessment of topical drug bioavailability. Herkenne C, Alberti I, Naik A, Kalia YN, Mathy FX, Préat V, Guy RH. School of Pharmaceutical Sciences, University of Geneva, 30 quai E. Ansermet, CH-1211, Geneva 4, Switzerland. This paper reviews some current methods for the in vivo assessment of local cutaneous bioavailability in humans after topical drug application. After an introduction discussing the importance of local drug bioavailability assessment and the limitations of model-based predictions, the focus turns to the relevance of experimental studies. The available techniques are then reviewed in detail, with particular emphasis on the tape stripping and microdialysis methodologies. Other less developed techniques, including the skin biopsy, suction blister, follicle removal and confocal Raman spectroscopy techniques are also described. PMID: 17985216 [PubMed - in process] Related Links In vivo microdialysis for the investigation of drug levels in the dermis and the effect of barrier perturbation on cutaneous drug penetration. Studies in hairless rats and human subjects. [Acta Derm Venereol Suppl (Stockh). 1999] PMID:10605601 In vivo assessment of the cutaneous bioavailability of topically applied maxacalcitol. [Methods Find Exp Clin Pharmacol. 2005] PMID:16082417 The role of stratum corneum and dermal microvascular perfusion in penetration and tissue levels of water-soluble drugs investigated by microdialysis. [Br J Dermatol. 2003] PMID:12653734 Assessment of topical bioavailability in vivo: the importance of stratum corneum thickness. [Skin Pharmacol Appl Skin Physiol. 2001] PMID:11509911 Progress in methodologies for evaluating bioequivalence of topical formulations. [Am J Clin Dermatol. 2001] PMID:11721645