ARTICLE AUTHOR: Gilliard-JA; Ritter-C

REPRINT AUTHOR: Gilliard, JA; QUAL ASSISTANCE SPRL; TECHNOPARC THUDINIE 1; B-6536 DONSTIENNES; BELGIUM

SOURCE: JOURNAL-OF-CHROMATOGRAPHY-A. JAN 10 1997; 758 (1) : 1-18

ABSTRACT:

The application of mixture analysis techniques to liquid chromatography-diode array detector (LC-DAD) data can be hampered by instrumental artefacts. If not properly accounted for, these artefacts may create major problems in the interpretation of the results by introducing additional factors in the analysis that can be difficult to distinguish from minor chemical components. In this paper, we show how the main sources of instrumental errors can be identified and how their effect can be mimicked by a specifically designed simulation procedure. Such realistic simulations are then successfully used to anticipate the expected response for an homogeneous peak even under non-ideal conditions, and hence to facilitate unequivocal interpretation of results.