Accurate and sensitive measurements of pO2 in vivo using low frequency EPR spectroscopy: how to confer biocompatibility to the oxygen sensors

Bernard Gallez * 1 * gallez@cmfa.ucl.ac.be and Karsten Mäder  2

* Laboratory of Medicinal Chemistry and Radiopharmacy and Laboratory of Biomedical Magnetic Resonance, Université Catholique de Louvain, Brussels, Belgium; Department of Pharmaceutical Sciences, Freie Universität, Berlin, Germany

Received 16 December 1999; received in revised form 10 May 2000; accepted 1 June 2000

Abstract

Within the last few years, there has been a significant amount of progress using EPR oximetry, which has resulted in the availability of instrumentation and paramagnetic materials capable of measuring pO2 in tissues with an accuracy and sensitivity comparable to or greater than that available by any other method. While the results obtained with EPR so far indicate that criteria for the measurements of pO2such as accuracy, sensitivity, repeatability, and noninvasivenesscan be met, some of the paramagnetic materials with optimum spectroscopic properties (i.e., strong simple signals which are appropriately responsive to changes in pO2) may have some undesirable interactions with tissues, causing reactions with and/or losing responsiveness to oxygen. In this paper, several approaches are discussed, such as encapsulation procedures, which can result in the availability of oxygen-sensitive materials in a suitable configuration for long-term studies (absence of toxicity and preservation of the responsiveness to oxygen).

Keywords: EPR; ESR; In vivo; Oxygen; Encapsulation; Free radicals

Abbreviations: EPR, Electron Paramagnetic Resonance; pO2, partial pressure of oxygen; w/w,
weight/weight