Checking the integrity of albumin after pasteurisation: a fluorescent assay for quantitating monomeric albumin using anthroyloxy-fatty acids analogs.

Gallez B, Lambert DM

Laboratory of Medicinal Chemistry and Radiopharmacy, Catholic University of Louvain, Brussels, Belgium. Gallez@cmfa.ucl.ac.be

This study presents a new assay based on the binding of anthroyloxy-stearates to albumin and the change in the fluorescence intensity when these fluorescent probes are bound to the protein. This assay permits the quantification of the albumin monomers present in a mixture of monomers and polymers, and allows to check the integrity of albumin after pasteurisation.

PMID: 9825296, UI: 99042737