1. Int J Pharm. 2010 Jul 15;394(1-2):35-42. Epub 2010 Apr 24.

In vitro identification of targeting ligands of human M cells by phage display.

Fievez V, Plapied L, Plaideau C, Legendre D, des Rieux A, Pourcelle V, Freichels 
H, Jérôme C, Marchand J, Préat V, Schneider YJ.

Université Catholique de Louvain, Institut des Sciences de la Vie, Biochimie
Cellulaire, Nutritionnelle & Toxicologique, Croix du Sud, 5, 1348
Louvain-La-Neuve, Belgium.

To improve transport of vaccine-loaded nanoparticles, the phage display
technology was used to identify novel lead peptides targeting human M cells.
Using an in vitro model of the human follicle-associated epithelium (FAE) which
contains both Caco-2 and M cells, a T7 phage display library was screened for its
ability either to bind the apical cell surface of or to undergo transcytosis
across Caco-2 cells or FAE. The selection for transcytosis across both
enterocytes and FAE identified three different peptide sequences (CTGKSC, PAVLG
and LRVG) with high frequency. CTGKSC and LRVG sequences enhanced phage transport
across M-like cells. When polymeric nanoparticles were grafted with the sequences
CTGKSC and LRVG, their transport by FAE was significantly enhanced. These
peptides could therefore be used to enhance the transport of vaccine-loaded
nanoparticles across the intestinal mucosal barrier. Copyright (c) 2010 Elsevier 
B.V. All rights reserved.

PMID: 20417702 [PubMed - in process]