1: Arch Toxicol  2001 Oct;75(8):452-8

Effect of cold hypoxic storage and reoxygenation at 37 degrees C of cultured
precision-cut rat liver slices on paracetamol metabolism.

Evdokimova E, Taper H, Buc Calderon P.

Departement de Sciences Pharmaceutiques, Universite Catholique de Louvain,
Brussels, Belgium.

The influence of cold storage and reoxygenation at 37 degrees C of precision-cut
rat liver slices on paracetamol metabolism was studied. A depressed metabolism
was observed after cold preservation, but during reoxygenation at 37 degrees C
slices were capable of synthesizing proteins and maintaining both glutathione
and ATP levels. Recovery was faster in slices under aerobic cold conditions than
in those under cold hypoxia. Glycogen levels were dramatically decreased under
both conditions and subsequent reoxygenation at 37 degrees C still increased the
glycogenolysis. Xenobiotic metabolism after reoxygenation of cold-preserved
slices shows that glucuronide and sulfate conjugates of paracetamol represent
about 50% of those of fresh slices at zero time. The amounts of phase II
apoproteins were virtually unchanged, thus suggesting that loss of enzyme
activities are most probably due to lack of cofactors. Reoxygenation at 37
degrees C did not impair cell metabolism, and a potential role for nitric oxide
and other cytokines released form Kupffer cells appears unlikely since nitrite
was not formed after bacterial endotoxin stimulation. The maintenance of
energetic stores during cold preservation appears, therefore, to be a critical
issue for the survival and metabolic activity of cells.

PMID: 11757668 [PubMed - in process]