In vitro percutaneous penetration through hairless rat skin: influence
of temperature, vehicle and penetration enhancers.
Clarys P, Alewaeters K, Jadoul A, Barel A, Manadas RO, Preat V
General and Biological Chemistry, Faculty of Physical Education and Physiotherapy, Vrije Universiteit Brussel, Brussels, Belgium. pclarys@vub.ac.be
Theoretically, a positive relation is expected between skin temperature
and the percutaneous penetration of topically applied substances. The aim
of the present study was to evaluate the influence of the temperature on
the in vitro percutaneous penetration of dihydrotestosterone. Hairless
rat skin was mounted in static diffusion cells placed in a water bath at
different temperatures (28.6, 35.1 and 38.2 degrees C, respectively). Different
vehicles were tested as well as the addition of penetration promoting molecules
such as oleic acid and limonene. A saline buffer was used as the receptor
phase. Penetration through the skin was evaluated by means of scintillation
counting of the radiolabelled dihydrotestosterone. Experiments were followed
for a period of 29 h. The total amount of penetrant, dihydrotestosterone,
as well as the flux, were calculated from these kinetics. Our results demonstrate
a temperature effect with the highest penetration at 38.2 degrees C. The
vehicle was also found to influence the penetration of dihydrotestosterone
in a significant way. Furthermore, limonene presented better penetration
promoting properties compared with oleic acid. Copyright 1998 Elsevier
Science B.V.
PMID: 9885299, UI: 99104291