1: Magn Reson Med. 2006 Jan;55(1):215-8. 

Evaluation of lipid-based carrier systems and inclusion complexes of
diethyldithiocarbamate-iron to trap nitric oxide in biological systems.

Charlier N, Preat V, Gallez B.

Laboratory of Medicinal Chemistry and Radiopharmacy, Biomedical Resonance Unit,
Universite Catholique de Louvain, Brussels, Belgium.

The success of spin trapping techniques in vivo hinges on whether spin traps
with high trapping efficiency and biocompatibility can be developed. Currently,
two iron chelates based on the dithiocarbamate structure (hydrophilic
ferro-di(N-methyl-D-glucamine-dithiocarbamate, or Fe(II)-MGD, and lipophilic
ferro-di(diethyldithiocarbamate), or Fe(II)-DETC), are used for spin trapping of
nitric oxide (NO) in biologic systems. However, detection efficiency is hampered
by a complex redox chemistry for Fe(II)-MGD and by the insolubility of
Fe(II)-DETC in water. To circumvent these problems, two new spin trap
formulations based on Fe(II)-DETC were developed: a lipid-based carrier system
stabilized by lecithin and inclusion complexes in
hydroxypropyl-beta-cyclodextrin. The capability of these two systems to trap NO
was determined and compared to the standard spin traps in vitro (in the presence
of an NO donor) and in vivo (after induction of septic shock in mice). The
sensitivity of the detection of NO was significantly increased (by a factor of
4) using the lipid-based carrier systems or inclusion complexes compared to the
standard spin trap agents.

PMID: 16342156 [PubMed - in process]