1: J Endocrinol. 2005 Jun;185(3):457-65. 

Involvement of endogenous glucagon-like peptide-1(7-36) amide on
glycaemia-lowering effect of oligofructose in streptozotocin-treated rats.

Cani PD, Daubioul CA, Reusens B, Remacle C, Catillon G, Delzenne NM.

Unit of Pharmacokinetics, Metabolism, Nutrition and Toxicology, Universite
catholique de Louvain, Brussels, Belgium.

We have evaluated the influence of oligofructose (OFS), a fermentable dietary
fibre, on glucose homeostasis, insulin production and intestinal glucagon-like
peptide-1 (GLP-1) in streptozotocin-treated diabetic rats. Male Wistar rats
received either i.v. streptozotocin (STZ; 40 mg/kg) or vehicle (CT); one week
later, they were fed for 6 weeks with either the standard diet (STZ-CT), or with
a diet containing 10% oligofructose (STZ-OFS); both diets were available ad
libitum. In a second set of experiments (duration 4 weeks), a supplemental group
of food-restricted rats (STZ-Res) receiving a similar intake as CT rats, was
added. OFS improved glucose tolerance and reduced food intake as compared with
STZ-CT rats in both the post-prandial state and after an oral glucose tolerance
test. After 6 weeks, portal and pancreatic insulin concentrations were doubled
in STZ-OFS rats. Food restriction improved these parameters when compared with
STZ-CT rats, but to a lesser extent than in the STZ-OFS group. We have shown
that OFS treatment increased portal and colonic GLP-1(7-36) amide levels and
doubled colonic proglucagon and prohormone convertase 1 mRNA levels; both OFS
and food restriction lowered ileal GLP-1(7-36) amide levels as compared with
levels in STZ-CT rats. We propose that OFS, through its fermentation in the
colon, promotes the expression and secretion of colonic peptides, namely
GLP-1(7-36) amide, with beneficial consequences on glycaemia, insulin secretion
and hyperphagia in diabetic rats.

PMID: 15930172 [PubMed - indexed for MEDLINE]