Saccharomyces boulardii upgrades cellular adaptation after proximal
enterectomy in rats.
Buts JP, De Keyser N, Marandi S, Hermans D, Sokal EM, Chae YH, Lambotte
L, Chanteux H, Tulkens PM
Laboratory of Paediatric Gastroenterology and Nutrition, Faculty of Medicine, Catholic University of Louvain, Brussels, Belgium.
BACKGROUND: Saccharomyces boulardii is a non-pathogenic yeast which
exerts trophic effects on human and rat small intestinal mucosa. AIMS:
To examine the effects of S boulardii on ileal adaptation after proximal
enterectomy in rats. METHODS: Wistar rats, aged eight weeks, underwent
60% proximal resection or transection and received by orogastric intubation
either 1 mg/g body wt per day lyophilised S boulardii or the vehicle for
seven days. The effects on ileal mucosal adaptation were assessed eight
days after surgery. RESULTS: Compared with transection, resection resulted
in mucosal hyperplasia with significant decreases in the specific and total
activities of sucrase, lactase, and maltase. Treatment of resected animals
with S boulardii had no effect on mucosal yperplasia but did upgrade disaccharidase
activities to the levels of the transected group. Enzyme stimulation by
S boulardii was associated with significant increases in diamine oxidase
activity and mucosal polyamine concentrations. Likewise, sodium dependent
D-glucose uptake by brush border membrane vesicles, measured as a function
of time and glucose concentration in the incubation medium, was significantly
(p<0.05) increased by 81% and three times respectively in the resected
group treated with S boulardii. In agreement with this, expression of the
sodium/glucose cotransporter-1 in brush border membranes of resected rats
treated with S boulardii was enhanced twofold compared with resected controls.
CONCLUSION: Oral administration of S boulardii soon after proximal enterectomy
improves functional adaptation of the remnant ileum.
PMID: 10369710, UI: 99298222