1: FEBS Lett. 2008 Nov 26;582(28):3861-7. Epub 2008 Oct 23.

Reciprocal influences of CB1 cannabinoid receptor agonists on ERK and JNK
signalling in N1E-115 cells.

Bosier B, Lambert DM, Hermans E.

Unité de Chimie Pharmaceutique et de Radiopharmacie (UCL 7340), Université
catholique de Louvain, 73 40, Av E.Mounier, B-1200 Brussels, Belgium.

Agonists acting at the CB1 cannabinoid receptor in N1E-115 neuroblastoma cells
were found to activate MAPK family members with reciprocal efficacies. Thus, HU
210 robustly increased phosphorylation of ERK1/2 whereas CP 55,940 was more
effective in activating JNK. The use of selected kinase inhibitors confirmed that
distinct signalling cascades were involved in these responses. This reciprocal
control of MAPK activity was correlated with the observation that HU 210- and CP 
55,940-mediated regulations of tyrosine hydroxylase gene expression were
respectively impaired by MEK and JNK inhibitors. These data indicate that complex
interactions of the CB1 receptor with intracellular signalling partners
controlling MAPK activities may explain the apparent disparities in cellular
responses to functional selective agonists.


PMID: 18950629 [PubMed - indexed for MEDLINE]

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