1: Phytochem Anal. 2005 Sep-Oct;16(5):342-8.

A validated method for the quantification of pimarane and trachylobane
diterpenes in the leaves of Croton zambesicus by capillary gas chromatography.

Block S, Brkic D, Hubert P, Quetin-Leclercq J.

Laboratoire de Pharmacognosie, Unite d'Analyse Chimique et Physico-Chimique des
Medicaments, Universite Catholique de Louvain, Bruxelles, Belgium.

A sensitive and accurate method, combining Soxhlet extraction, solid-phase
extraction and capillary gas chromatography, is described for the quantitative
determination of four new diterpenes (ent-trachyloban-3beta-ol,
ent-18-hydroxy-trachyloban-3-one, ent-trachyloban-3-one and
isopimara-7,15-dien-3beta-ol) from the leaves of Croton zambesicus. This is the
first method describing the quantification of trachylobane diterpenes in a crude
extract. It has been fully validated in order to be able to compare the
diterpene composition in other samples of C. zambesicus, which is an important
source of trachylobanes.

PMID: 16223091 [PubMed - indexed for MEDLINE]

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