Planta Med 2002 Jul;68(7):647-9 ent-trachyloban-3beta-ol, a new cytotoxic diterpene from Croton zambesicus. Block S, Stevigny C, De Pauw-Gillet MC, de Hoffmann E, Llabres G, Adjakidje V, Quetin-Leclercq J. The dichloromethane extract of leaves of Croton zambesicus (Euphorbiaceae) showing in vitro cytotoxicity against human cervix carcinoma cells was investigated in order to identify its active compounds. A bio-guided fractionation by HSCCC followed by MPLC led us to isolate a trachylobane diterpene, ent-trachyloban-3beta-ol, with cytotoxic properties (IC50 on HeLa cells = 7.3 microg/ml). This is the first report on the cytotoxicity of a trachylobane diterpene. Publication Types: Letter PMID: 12143003 [PubMed - indexed for MEDLINE]